Pro veritate
in vitro
For truth in glass
The
The independent review of research-grade reconstitution practice
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R. Calloway
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Step-by-step reconstitution, storage, and handling protocols. Each cites the underlying USP chapter or peer-reviewed source.
Protocols
June 7, 2026It's not a marketing number — it's the validated outer edge of the USP <51> preservative effectiveness window
Every credible source says 'discard after 28 days post-puncture, refrigerated.' Few sources explain why. Here's the underlying USP <51> validation logic and what changes if you ignore the rule.
By R. Calloway · 5 min
Protocols
May 22, 2026What to actually do with the vial between manufacture and disposal
Bacteriostatic water typically arrives with a 2-year shelf life under proper storage conditions. The Certificate of Analysis for a recent batch from BAC Water Depot batch number BW20230101 specifies storage at 15-30 °C, away from direct ...
By R. Calloway · 2 min
Protocols
May 17, 2026A walkthrough of the math, with examples for 5 mg and 10 mg peptide vials
When reconstituting research peptides, the most common mistake is using too little diluent, resulting in concentrations that are higher than intended. A 5 mg peptide vial reconstituted with 1 mL of diluent yields 5 mg/mL—twice the target...
By R. Calloway · 2 min
Protocols
May 17, 2026What 'aseptic' actually means in a research lab without a biosafety cabinet
A recent survey of our readers revealed that 1 in 5 labs do not follow proper aseptic technique when drawing from multi-dose vials, with 30% of respondents admitting to reusing needles between draws. The USP <71> sterility test req...
By R. Calloway · 2 min